Biotechnology edition expanding in mass role second spectrometry

To do so, she has developed a group of antibodies reactive against a variety of protein modification sites, such as phosphorylation, nitration, acetylation, and substrates of a specific modification enzyme, etc. Among those, phospho-specific antibodies have enabled isolation of a large number of phosphorylated peptides that can be subsequently identified by tandem mass spectrometry. A second technology enables capturing glycopeptides using solid phase extraction, which has become a powerful tool to analyze glycoproteins on cell surface and in body fluids.

Thus far, thousands of novel glycosylation sites have been identified from different tissues using this novel glycopeptide capture technology; this significantly expends the limited number of glycosylation sites experimentally identified prior to the new technology. These methods are highly sensitive, holding a strong promise for discovering low abundance disease marker proteins in tissue, plasma or other body fluids. Currently, Dr. Zhang is applying these proteomics technologies to determine protein modifications associated with cancer, which well help for early detection and improved monitoring of therapeutic effects.

She is also developing novel methods to study protein modifications that will have major implications for a wide range of health issues. Deep proteogenomic characterization of human ovarian cancer. Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nature Biotechnology. It is not a specialist book, as that is intended by the authors I believe biotechnology, the text mentions, is a huge multidisciplinary field.

What it was good for was bridging my recent microbiology and chemistry exposure and showing me applications in the real world. It also gives a good image of possibilities and current limitations, as well as areas of research that I may potentially pursue. While this was great, I have to deduct some points for the lack of concrete data contained in the textbook. It was clearly written for someone with minimal exposure to technical mathematics or statistics, perhaps for a first year student of biological sciences.

That is both a blessing and a curse, as the takeaway from the text itself is a little nebulous. It certainly lacks any tangible biophysics component. Perhaps that was the intent, but it certainly makes me hanker for a little more data driven deliveries. Add to cart.

Sales tax will be calculated at check-out. Free Global Shipping. Chapter 10, which completes the book, presents research that may give a glimpse of the future: measuring the mass of intact viruses. Because viruses are generally structurally well-defined entities, mass measurements are potentially useful for viral identification. Interestingly, it has been shown that ESI followed by MS is gentle enough for some viruses to retain virulence, indicating that the mass spectrometer measures the clinically interesting object, the intact virus.

A section on terms and definitions follows the last chapter and contains a subset of MS terms and definitions. Although not fully comprehensive, it contains most of the terminology of interest to the target audience. In a few cases the information is incomplete or contains typographic errors.

In at least one case the author conforms to accepted usage, e. This should not be construed as a criticism of the author, but rather a criticism of inconsistencies in the field itself, which have not yet been satisfactorily resolved.

Although the book is very well written, it is marred somewhat by numerous typographic and other errors. In most cases these do not obscure the meaning, but they are at times distracting. To take just one example, there are numerous errors in notation, such as appearing in place of 10 6. Additionally, there are a few items with which other mass spectrometrists may disagree, such as the statement in Table 1. To summarize, this very readable book packs a large amount of information in a relatively small number of pages.

I recommend it as a good survey of MS methods related to biotechnology. Although not emphasizing clinical applications, there is enough information in the book related to these or that could be adapted to clinical applications to make the book worth reading by clinical chemists, particularly because the field it does emphasize, biomedical research, is of great interest.

Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Some mass spectrometrists use the definition that is similar to definitions used in some other fields of physics and chemistry. In this case, resolving power is defined as:. Resolution and resolving power, when defined in this way, are consistent with IUPAC recommendations for microscopy , optical spectroscopy.

The two most widely used are the peak width definition and the valley definition. The latter is called the full width at half maximum FWHM. From Infogalactic: the planetary knowledge core. Jump to: navigation , search. This article is about the quantity used in mass spectrometry.

For other uses, see Resolution disambiguation. Online corrected version: — " resolution in mass spectroscopy ". Online corrected version: — " resolving power in mass spectrometry ". Mass Spectrometry: Organic Chemical Applications. New York: McGraw-Hill.